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donkey α mouse cy3 antibody  (Jackson Immuno)


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    Structured Review

    Jackson Immuno donkey α mouse cy3 antibody
    A) RT-qPCR analysis of indicated transcripts in isolated fat bodies from female animals expressing UAS transgenes encoding control ( LacZ RNAi ), ATF4 RNAi or EcR RNAi . UAS -transgene expression is driven by Dcg-GAL4 . Data represent average of four biological replicates and error bars represent standard error of mean. B) Representative confocal images showing 4E-BP intron -DsRed reporter expression (magenta) in female fat bodies also expressing indicated RNAi lines driven by Dcg-GAL4 . Nuclei are counterstained with DAPI (cyan). C) Quantification of nuclear DsRed in individual adipocytes from B . Data represent mean from at least 18 animals collected from three independent crosses. The dotted line indicates the average of the control ( LacZ RNAi ) sample. D) Relative Change in FRET after Acceptor (ATF4-GFP labeled with Cy5) photobleach upon excitation of the donor (EcR labeled with <t>Cy3)</t> in the nuclei of adipocytes from control ( w 1118 ) or ATF4-GFP expressing ( crc GFSTF /+ ) female animals. Data represent mean from at least 18 animals collected from three independent crosses. The dotted line indicates the average of the control ( w 1118 ) sample. Scale bar=50 μm. ****=p<0.00001; ***=p<0.0001; **=p<0.001; *=p<0.05. Only significant comparisons are shown. Statistical tests are described in the methods.
    Donkey α Mouse Cy3 Antibody, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 96/100, based on 1881 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/donkey α mouse cy3 antibody/product/Jackson Immuno
    Average 96 stars, based on 1881 article reviews
    donkey α mouse cy3 antibody - by Bioz Stars, 2026-06
    96/100 stars

    Images

    1) Product Images from "ATF4 and EcR interact to mediate both transcriptional activation and repression in the Drosophila fat body"

    Article Title: ATF4 and EcR interact to mediate both transcriptional activation and repression in the Drosophila fat body

    Journal: bioRxiv

    doi: 10.64898/2026.03.10.710866

    A) RT-qPCR analysis of indicated transcripts in isolated fat bodies from female animals expressing UAS transgenes encoding control ( LacZ RNAi ), ATF4 RNAi or EcR RNAi . UAS -transgene expression is driven by Dcg-GAL4 . Data represent average of four biological replicates and error bars represent standard error of mean. B) Representative confocal images showing 4E-BP intron -DsRed reporter expression (magenta) in female fat bodies also expressing indicated RNAi lines driven by Dcg-GAL4 . Nuclei are counterstained with DAPI (cyan). C) Quantification of nuclear DsRed in individual adipocytes from B . Data represent mean from at least 18 animals collected from three independent crosses. The dotted line indicates the average of the control ( LacZ RNAi ) sample. D) Relative Change in FRET after Acceptor (ATF4-GFP labeled with Cy5) photobleach upon excitation of the donor (EcR labeled with Cy3) in the nuclei of adipocytes from control ( w 1118 ) or ATF4-GFP expressing ( crc GFSTF /+ ) female animals. Data represent mean from at least 18 animals collected from three independent crosses. The dotted line indicates the average of the control ( w 1118 ) sample. Scale bar=50 μm. ****=p<0.00001; ***=p<0.0001; **=p<0.001; *=p<0.05. Only significant comparisons are shown. Statistical tests are described in the methods.
    Figure Legend Snippet: A) RT-qPCR analysis of indicated transcripts in isolated fat bodies from female animals expressing UAS transgenes encoding control ( LacZ RNAi ), ATF4 RNAi or EcR RNAi . UAS -transgene expression is driven by Dcg-GAL4 . Data represent average of four biological replicates and error bars represent standard error of mean. B) Representative confocal images showing 4E-BP intron -DsRed reporter expression (magenta) in female fat bodies also expressing indicated RNAi lines driven by Dcg-GAL4 . Nuclei are counterstained with DAPI (cyan). C) Quantification of nuclear DsRed in individual adipocytes from B . Data represent mean from at least 18 animals collected from three independent crosses. The dotted line indicates the average of the control ( LacZ RNAi ) sample. D) Relative Change in FRET after Acceptor (ATF4-GFP labeled with Cy5) photobleach upon excitation of the donor (EcR labeled with Cy3) in the nuclei of adipocytes from control ( w 1118 ) or ATF4-GFP expressing ( crc GFSTF /+ ) female animals. Data represent mean from at least 18 animals collected from three independent crosses. The dotted line indicates the average of the control ( w 1118 ) sample. Scale bar=50 μm. ****=p<0.00001; ***=p<0.0001; **=p<0.001; *=p<0.05. Only significant comparisons are shown. Statistical tests are described in the methods.

    Techniques Used: Quantitative RT-PCR, Isolation, Expressing, Control, Labeling

    A) Representative confocal images showing 4E-BP intron -GFP reporter expression (magenta) in fat bodies also expressing LacZ RNAi , either ATF4 RNAi or usp RNAi , and both usp RNAi and ATF4 RNAi driven by Dcg-GAL4. Equal number of UAS-transgene cassettes were used by combining RNAi lines with either LacZ or LacZ RNAi . B) Quantification of nuclear GFP in individual adipocytes from A . Data represent mean from at least 18 animals collected from three independent crosses. The dotted line indicates the average of the control ( LacZ RNAi ) sample. C) Relative Change in FRET after Acceptor (Usp-GFP labeled with Cy5) photobleach upon excitation of the donor (EcR labeled with Cy3) in the nuclei of wandering third instar larval fat bodies where Dcg-GAL4 drives expression of control ( LacZ RNAi ) or ATF4 RNAi . Data represent mean from at least 18 animals collected from three independent crosses. The dotted line indicates the average of the control ( LacZ RNAi ) sample. Scale bar=50 μm. ****=p<0.00001; ***=p<0.0001; **=p<0.001; *=p<0.05. Only significant comparisons are shown. Statistical tests are described in the methods.
    Figure Legend Snippet: A) Representative confocal images showing 4E-BP intron -GFP reporter expression (magenta) in fat bodies also expressing LacZ RNAi , either ATF4 RNAi or usp RNAi , and both usp RNAi and ATF4 RNAi driven by Dcg-GAL4. Equal number of UAS-transgene cassettes were used by combining RNAi lines with either LacZ or LacZ RNAi . B) Quantification of nuclear GFP in individual adipocytes from A . Data represent mean from at least 18 animals collected from three independent crosses. The dotted line indicates the average of the control ( LacZ RNAi ) sample. C) Relative Change in FRET after Acceptor (Usp-GFP labeled with Cy5) photobleach upon excitation of the donor (EcR labeled with Cy3) in the nuclei of wandering third instar larval fat bodies where Dcg-GAL4 drives expression of control ( LacZ RNAi ) or ATF4 RNAi . Data represent mean from at least 18 animals collected from three independent crosses. The dotted line indicates the average of the control ( LacZ RNAi ) sample. Scale bar=50 μm. ****=p<0.00001; ***=p<0.0001; **=p<0.001; *=p<0.05. Only significant comparisons are shown. Statistical tests are described in the methods.

    Techniques Used: Expressing, Control, Labeling



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    Image Search Results


    A) RT-qPCR analysis of indicated transcripts in isolated fat bodies from female animals expressing UAS transgenes encoding control ( LacZ RNAi ), ATF4 RNAi or EcR RNAi . UAS -transgene expression is driven by Dcg-GAL4 . Data represent average of four biological replicates and error bars represent standard error of mean. B) Representative confocal images showing 4E-BP intron -DsRed reporter expression (magenta) in female fat bodies also expressing indicated RNAi lines driven by Dcg-GAL4 . Nuclei are counterstained with DAPI (cyan). C) Quantification of nuclear DsRed in individual adipocytes from B . Data represent mean from at least 18 animals collected from three independent crosses. The dotted line indicates the average of the control ( LacZ RNAi ) sample. D) Relative Change in FRET after Acceptor (ATF4-GFP labeled with Cy5) photobleach upon excitation of the donor (EcR labeled with Cy3) in the nuclei of adipocytes from control ( w 1118 ) or ATF4-GFP expressing ( crc GFSTF /+ ) female animals. Data represent mean from at least 18 animals collected from three independent crosses. The dotted line indicates the average of the control ( w 1118 ) sample. Scale bar=50 μm. ****=p<0.00001; ***=p<0.0001; **=p<0.001; *=p<0.05. Only significant comparisons are shown. Statistical tests are described in the methods.

    Journal: bioRxiv

    Article Title: ATF4 and EcR interact to mediate both transcriptional activation and repression in the Drosophila fat body

    doi: 10.64898/2026.03.10.710866

    Figure Lengend Snippet: A) RT-qPCR analysis of indicated transcripts in isolated fat bodies from female animals expressing UAS transgenes encoding control ( LacZ RNAi ), ATF4 RNAi or EcR RNAi . UAS -transgene expression is driven by Dcg-GAL4 . Data represent average of four biological replicates and error bars represent standard error of mean. B) Representative confocal images showing 4E-BP intron -DsRed reporter expression (magenta) in female fat bodies also expressing indicated RNAi lines driven by Dcg-GAL4 . Nuclei are counterstained with DAPI (cyan). C) Quantification of nuclear DsRed in individual adipocytes from B . Data represent mean from at least 18 animals collected from three independent crosses. The dotted line indicates the average of the control ( LacZ RNAi ) sample. D) Relative Change in FRET after Acceptor (ATF4-GFP labeled with Cy5) photobleach upon excitation of the donor (EcR labeled with Cy3) in the nuclei of adipocytes from control ( w 1118 ) or ATF4-GFP expressing ( crc GFSTF /+ ) female animals. Data represent mean from at least 18 animals collected from three independent crosses. The dotted line indicates the average of the control ( w 1118 ) sample. Scale bar=50 μm. ****=p<0.00001; ***=p<0.0001; **=p<0.001; *=p<0.05. Only significant comparisons are shown. Statistical tests are described in the methods.

    Article Snippet: Samples were incubated for 1 hour in the dark in donkey α-mouse Cy3 antibody (1:1000, Jackson ImmunoResearch #715-165-150) and donkey α-chicken Cy5 antibody (1:1000, Jackson ImmunoResearch #703-175-155) diluted in PBS.

    Techniques: Quantitative RT-PCR, Isolation, Expressing, Control, Labeling

    A) Representative confocal images showing 4E-BP intron -GFP reporter expression (magenta) in fat bodies also expressing LacZ RNAi , either ATF4 RNAi or usp RNAi , and both usp RNAi and ATF4 RNAi driven by Dcg-GAL4. Equal number of UAS-transgene cassettes were used by combining RNAi lines with either LacZ or LacZ RNAi . B) Quantification of nuclear GFP in individual adipocytes from A . Data represent mean from at least 18 animals collected from three independent crosses. The dotted line indicates the average of the control ( LacZ RNAi ) sample. C) Relative Change in FRET after Acceptor (Usp-GFP labeled with Cy5) photobleach upon excitation of the donor (EcR labeled with Cy3) in the nuclei of wandering third instar larval fat bodies where Dcg-GAL4 drives expression of control ( LacZ RNAi ) or ATF4 RNAi . Data represent mean from at least 18 animals collected from three independent crosses. The dotted line indicates the average of the control ( LacZ RNAi ) sample. Scale bar=50 μm. ****=p<0.00001; ***=p<0.0001; **=p<0.001; *=p<0.05. Only significant comparisons are shown. Statistical tests are described in the methods.

    Journal: bioRxiv

    Article Title: ATF4 and EcR interact to mediate both transcriptional activation and repression in the Drosophila fat body

    doi: 10.64898/2026.03.10.710866

    Figure Lengend Snippet: A) Representative confocal images showing 4E-BP intron -GFP reporter expression (magenta) in fat bodies also expressing LacZ RNAi , either ATF4 RNAi or usp RNAi , and both usp RNAi and ATF4 RNAi driven by Dcg-GAL4. Equal number of UAS-transgene cassettes were used by combining RNAi lines with either LacZ or LacZ RNAi . B) Quantification of nuclear GFP in individual adipocytes from A . Data represent mean from at least 18 animals collected from three independent crosses. The dotted line indicates the average of the control ( LacZ RNAi ) sample. C) Relative Change in FRET after Acceptor (Usp-GFP labeled with Cy5) photobleach upon excitation of the donor (EcR labeled with Cy3) in the nuclei of wandering third instar larval fat bodies where Dcg-GAL4 drives expression of control ( LacZ RNAi ) or ATF4 RNAi . Data represent mean from at least 18 animals collected from three independent crosses. The dotted line indicates the average of the control ( LacZ RNAi ) sample. Scale bar=50 μm. ****=p<0.00001; ***=p<0.0001; **=p<0.001; *=p<0.05. Only significant comparisons are shown. Statistical tests are described in the methods.

    Article Snippet: Samples were incubated for 1 hour in the dark in donkey α-mouse Cy3 antibody (1:1000, Jackson ImmunoResearch #715-165-150) and donkey α-chicken Cy5 antibody (1:1000, Jackson ImmunoResearch #703-175-155) diluted in PBS.

    Techniques: Expressing, Control, Labeling